TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp nfo用于末端检测，检测DNA扩增反应. 末端三明治法快速检测，如侧向流试纸法.除基本组分之外， nfo核酶，和适合是LF 探针 形成新的聚合酶增强底物，无需任何仪器。本品可通过加入exo探针作为Amp exo kit的替代物，进行荧光定量检测，由于核酶延缓动态反应速度，可用于末端电泳检测。

 Basic Information:

RPA

1) Primers must be 30-35 bases

2) Works best at constant temperature (37-39°C)

3) Amplicons of 80-400bp are preferred

4) TwistAmp® LF Probe or TwistAmp® exo Probe 1  required - see overleaf

Set-up (single-plex)2

1) Prepare reaction mix in 1.5ml tube:

Primer A (10μM)    2.1 μl

Primer B (10μM)    2.1 μl

TwistAmp® LF Probe (10μM)      0.6 μl

Rehydration Buffer   29.5 μl

Template and dH2O   13.2 μl

(Total Volume    47.5 μl)

Vortex and spin briefly

2) Add reaction mix to freeze-dried reaction. Pipette to mix.

3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.

4) Incubate at 37-39°C for 20-40 minutes.

4b) For low template copy number,remove strip after 4 minutes, vortex &spin briefly, replace in heating device.

5) For analysis by lateral flow, dilute reacion products 1/50 with PBST and load 10 μl onto the sample pad of appropriate

strip (eg Milenia® HybriDetect strips).

6) Place strip in PBST running buffer.

WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS

MAGNESIUM IS ADDED.

温馨提示：不可用于临床治疗。